A rainbow of fluoromodules: A promiscuous scFv protein binds to and activates a diverse set of fluorogenic cyanine dyes

Hayriye Özhalici-Ünal; Crystal Lee Pow; Sarah A. Marks; Lawrence D. Jesper; Gloria L. Silva; Nathaniel I. Shank; Elizabeth W. Jones; James M. Burnette; Peter B. Berget; Bruce A. Armitage

doi:10.1021/ja805042p

A rainbow of fluoromodules: A promiscuous scFv protein binds to and activates a diverse set of fluorogenic cyanine dyes

Hayriye Özhalici-Ünal, Crystal Lee Pow, Sarah A. Marks, Lawrence D. Jesper, Gloria L. Silva, Nathaniel I. Shank, Elizabeth W. Jones, James M. Burnette, Peter B. Berget, Bruce A. Armitage

Biochemistry, Chemistry, & Physics

Research output: Contribution to journal › Article › peer-review

93 Scopus citations

Abstract

Combined magnetic and fluorescence cell sorting were used to select Fluorogen Activating Proteins (FAPs) from a yeast surface-displayed library for binding to the fluorogenic cyanine dye Dimethyl Indole Red (DIR). Several FAPs were selected that bind to the dye with low nanomolar K_d values and enhance fluorescence more than 100-fold. One of these FAPs also exhibits considerable promiscuity, binding with high affinity to several other fluorogenic cyanine dyes with emission wavelengths covering most of the visible and near-IR regions of the spectrum. This significantly expands the number and wavelength range of scFv-based fluoromodules.

Original language	English
Pages (from-to)	12620-12621
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	130
Issue number	38
DOIs	https://doi.org/10.1021/ja805042p
State	Published - Sep 24 2008

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Özhalici-Ünal, H., Pow, C. L., Marks, S. A., Jesper, L. D., Silva, G. L., Shank, N. I., Jones, E. W., Burnette, J. M., Berget, P. B., & Armitage, B. A. (2008). A rainbow of fluoromodules: A promiscuous scFv protein binds to and activates a diverse set of fluorogenic cyanine dyes. Journal of the American Chemical Society, 130(38), 12620-12621. https://doi.org/10.1021/ja805042p

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abstract = "Combined magnetic and fluorescence cell sorting were used to select Fluorogen Activating Proteins (FAPs) from a yeast surface-displayed library for binding to the fluorogenic cyanine dye Dimethyl Indole Red (DIR). Several FAPs were selected that bind to the dye with low nanomolar Kd values and enhance fluorescence more than 100-fold. One of these FAPs also exhibits considerable promiscuity, binding with high affinity to several other fluorogenic cyanine dyes with emission wavelengths covering most of the visible and near-IR regions of the spectrum. This significantly expands the number and wavelength range of scFv-based fluoromodules.",

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year = "2008",

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AU - Özhalici-Ünal, Hayriye

AU - Pow, Crystal Lee

AU - Marks, Sarah A.

AU - Jesper, Lawrence D.

AU - Silva, Gloria L.

AU - Shank, Nathaniel I.

AU - Jones, Elizabeth W.

AU - Burnette, James M.

AU - Berget, Peter B.

AU - Armitage, Bruce A.

PY - 2008/9/24

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AB - Combined magnetic and fluorescence cell sorting were used to select Fluorogen Activating Proteins (FAPs) from a yeast surface-displayed library for binding to the fluorogenic cyanine dye Dimethyl Indole Red (DIR). Several FAPs were selected that bind to the dye with low nanomolar Kd values and enhance fluorescence more than 100-fold. One of these FAPs also exhibits considerable promiscuity, binding with high affinity to several other fluorogenic cyanine dyes with emission wavelengths covering most of the visible and near-IR regions of the spectrum. This significantly expands the number and wavelength range of scFv-based fluoromodules.

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A rainbow of fluoromodules: A promiscuous scFv protein binds to and activates a diverse set of fluorogenic cyanine dyes

Abstract

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