TY - JOUR
T1 - Ascidian-Inspired Heparin-Mimetic Magnetic Nanoparticles with Potential for Application in Hemodialysis as Recycling Anticoagulants
AU - Wang, Lingren
AU - Gong, Tao
AU - Brown, Zachary
AU - Randle, Christopher
AU - Guan, Yingying
AU - Ye, Wei
AU - Ming, Weihua
N1 - Publisher Copyright:
© 2020 American Chemical Society.
PY - 2020/4/13
Y1 - 2020/4/13
N2 - In the present study, heparin-mimetic magnetic nanoparticles (HMNPs), which might be used as recycling anticoagulants, were synthesized by coating heparin-mimetic sodium alginate (HLSA) on the surface of iron oxide magnetic nanoparticles (MNPs), using 3,4,5-trihydroxyphenylalanine (TOPA) as a biological adhesive. HLSA was successfully immobilized on the MNP surface, as revealed by Fourier transform infrared spectroscopy and thermal gravimetric analysis, and the core (MNP)-shell (TOPA, HLSA) structure was confirmed by transmission electron microscopy observations. In addition, in vitro studies of protein adsorption, blood clotting time, and contact activation confirmed that the blood compatibility of the HMNP was significantly enhanced compared with the bare MNP. The improved hemocompatibility was attributed to the introduction of the multiple heparin-mimetic groups (-SO3Na, -COONa, and -OH). In addition, the HMNP showed outstanding recycle stability and, thus, can be reused if needed. The synthesized HMNP appeared to be a suitable biomaterial to safely replace heparin as an anticoagulant in patients undergoing long-term hemodialysis.
AB - In the present study, heparin-mimetic magnetic nanoparticles (HMNPs), which might be used as recycling anticoagulants, were synthesized by coating heparin-mimetic sodium alginate (HLSA) on the surface of iron oxide magnetic nanoparticles (MNPs), using 3,4,5-trihydroxyphenylalanine (TOPA) as a biological adhesive. HLSA was successfully immobilized on the MNP surface, as revealed by Fourier transform infrared spectroscopy and thermal gravimetric analysis, and the core (MNP)-shell (TOPA, HLSA) structure was confirmed by transmission electron microscopy observations. In addition, in vitro studies of protein adsorption, blood clotting time, and contact activation confirmed that the blood compatibility of the HMNP was significantly enhanced compared with the bare MNP. The improved hemocompatibility was attributed to the introduction of the multiple heparin-mimetic groups (-SO3Na, -COONa, and -OH). In addition, the HMNP showed outstanding recycle stability and, thus, can be reused if needed. The synthesized HMNP appeared to be a suitable biomaterial to safely replace heparin as an anticoagulant in patients undergoing long-term hemodialysis.
KW - blood compatibility
KW - heparin-mimetic groups
KW - magnetic nanoparticles
KW - recyclability
UR - http://www.scopus.com/inward/record.url?scp=85083887298&partnerID=8YFLogxK
U2 - 10.1021/acsbiomaterials.9b01865
DO - 10.1021/acsbiomaterials.9b01865
M3 - Article
C2 - 33455351
AN - SCOPUS:85083887298
SN - 2373-9878
VL - 6
SP - 1998
EP - 2006
JO - ACS Biomaterials Science and Engineering
JF - ACS Biomaterials Science and Engineering
IS - 4
ER -