Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes

Susan E.H. West; Mary Jo M. Romero; Laura B. Regassa; Nicolette A. Zielinski; Rodney A. Welch

doi:10.1016/0378-1119(95)00236-Y

Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes

Susan E.H. West, Mary Jo M. Romero, Laura B. Regassa, Nicolette A. Zielinski, Rodney A. Welch

Biology

University of Wisconsin–Madison

Research output: Contribution to journal › Article › peer-review

38 Scopus citations

Abstract

We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the Km^R gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.

Original language	English
Pages (from-to)	81-86
Number of pages	6
Journal	Gene
Volume	160
Issue number	1
DOIs	https://doi.org/10.1016/0378-1119(95)00236-Y
State	Published - Jul 4 1995

Keywords

cloning
gene expression
Pasteurella haemolytica

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10.1016/0378-1119(95)00236-Y

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title = "Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes",

abstract = "We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.",

keywords = "cloning, gene expression, Pasteurella haemolytica",

author = "West, {Susan E.H.} and Romero, {Mary Jo M.} and Regassa, {Laura B.} and Zielinski, {Nicolette A.} and Welch, {Rodney A.}",

year = "1995",

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T1 - Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors

T2 - expression of antibiotic-resistance genes

AU - West, Susan E.H.

AU - Romero, Mary Jo M.

AU - Regassa, Laura B.

AU - Zielinski, Nicolette A.

AU - Welch, Rodney A.

PY - 1995/7/4

Y1 - 1995/7/4

N2 - We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.

AB - We constructed several cloning vectors, designated pGZRS-18/19 and pGZRS-38/39, which were based on an endogenous Actinobacillus pleuropneumoniae (Apl) 4.3-kb plasmid. They carry the lacZα-complementation fragment and MCS from pUC18/19, and either the bla gene under the control of a putative Apl promoter or the KmR gene from Tn903. These vectors replicate in representative strains of Apl serotypes 1 and 7, Escherichia coli, Pasteurella haemolytica (Ph) and Haemophilus (Actinobacillus) actinomycetemcomitans. We also found that Apl and Ph did not express genes under the control of the lacZ or bla promoters, suggesting that their RNA polymerases may not utilize these promoters.

KW - cloning

KW - gene expression

KW - Pasteurella haemolytica

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Construction of Actinobacillus pleuropneumoniae-Escherichia coli shuttle vectors: expression of antibiotic-resistance genes

Abstract

Keywords

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