Detection of Human Pathogenic Ehrlichia muris-like Agent in Peromyscus leucopus

Caroline G. Castillo; Marina E. Eremeeva; Susan M. Paskewitz; Lynne M. Sloan; Xia Lee; William E. Irwin; Stefan Tonsberg; Bobbi S. Pritt

doi:10.1016/j.ttbdis.2014.11.006

Detection of Human Pathogenic Ehrlichia muris-like Agent in Peromyscus leucopus

Caroline G. Castillo, Marina E. Eremeeva, Susan M. Paskewitz, Lynne M. Sloan, Xia Lee, William E. Irwin, Stefan Tonsberg, Bobbi S. Pritt

Biostatistics, Epidemiology & Environmental Health Sciences

Research output: Contribution to journal › Article › peer-review

21 Scopus citations

Abstract

An Ehrlichia muris-like (EML) bacterium was recently detected in humans and Ixodes scapularis ticks in Minnesota and Wisconsin. The reservoir for this agent is unknown. To investigate the occurrence of the EML agent, groEL PCR testing and sequencing was performed on blood from small mammals and white-tailed deer that were collected in areas where human and tick infections were previously demonstrated. DNA of the EML agent was detected in two Peromyscus leucopus of 146 small mammals (1.4%); while 181 O. virginianus tested negative. This report provides the first evidence that DNA from the EML agent is found in P. leucopus, the same animal that is a reservoir for Anaplasma phagocytophilum in this region. The role of white-tailed deer remains inconclusive. Further sampling is warranted to understand the spatial and temporal distribution, transmission and maintenance of this pathogen.

Original language	American English
Journal	Ticks and Tick-borne Diseases
DOIs	https://doi.org/10.1016/j.ttbdis.2014.11.006
State	Published - Jan 1 2014

Disciplines

Public Health
Environmental Public Health
Environmental Health and Protection

Keywords

Ehrlichia muris
Ehrlichia muris-like (EML) agent
Ehrlichiosis
Peromyscus

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.ttbdis.2014.11.006License: Unspecified

http://dx.doi.org/10.1016/j.ttbdis.2014.11.006

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@article{5d047401171c4e229013fbeb6585e87d,

title = "Detection of Human Pathogenic Ehrlichia muris-like Agent in Peromyscus leucopus",

abstract = "An Ehrlichia muris-like (EML) bacterium was recently detected in humans and Ixodes scapularis ticks in Minnesota and Wisconsin. The reservoir for this agent is unknown. To investigate the occurrence of the EML agent, groEL PCR testing and sequencing was performed on blood from small mammals and white-tailed deer that were collected in areas where human and tick infections were previously demonstrated. DNA of the EML agent was detected in two Peromyscus leucopus of 146 small mammals (1.4%); while 181 O. virginianus tested negative. This report provides the first evidence that DNA from the EML agent is found in P. leucopus, the same animal that is a reservoir for Anaplasma phagocytophilum in this region. The role of white-tailed deer remains inconclusive. Further sampling is warranted to understand the spatial and temporal distribution, transmission and maintenance of this pathogen.",

keywords = "Ehrlichia muris, Ehrlichia muris-like (EML) agent, Ehrlichiosis, Peromyscus",

author = "Castillo, {Caroline G.} and Eremeeva, {Marina E.} and Paskewitz, {Susan M.} and Sloan, {Lynne M.} and Xia Lee and Irwin, {William E.} and Stefan Tonsberg and Pritt, {Bobbi S.}",

note = "Publisher Copyright: {\textcopyright} 2014 Elsevier GmbH.",

year = "2014",

month = jan,

day = "1",

doi = "10.1016/j.ttbdis.2014.11.006",

language = "American English",

journal = "Ticks and Tick-borne Diseases",

issn = "1877-959X",

publisher = "Elsevier GmbH",

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TY - JOUR

T1 - Detection of Human Pathogenic Ehrlichia muris-like Agent in Peromyscus leucopus

AU - Castillo, Caroline G.

AU - Eremeeva, Marina E.

AU - Paskewitz, Susan M.

AU - Sloan, Lynne M.

AU - Lee, Xia

AU - Irwin, William E.

AU - Tonsberg, Stefan

AU - Pritt, Bobbi S.

PY - 2014/1/1

Y1 - 2014/1/1

N2 - An Ehrlichia muris-like (EML) bacterium was recently detected in humans and Ixodes scapularis ticks in Minnesota and Wisconsin. The reservoir for this agent is unknown. To investigate the occurrence of the EML agent, groEL PCR testing and sequencing was performed on blood from small mammals and white-tailed deer that were collected in areas where human and tick infections were previously demonstrated. DNA of the EML agent was detected in two Peromyscus leucopus of 146 small mammals (1.4%); while 181 O. virginianus tested negative. This report provides the first evidence that DNA from the EML agent is found in P. leucopus, the same animal that is a reservoir for Anaplasma phagocytophilum in this region. The role of white-tailed deer remains inconclusive. Further sampling is warranted to understand the spatial and temporal distribution, transmission and maintenance of this pathogen.

AB - An Ehrlichia muris-like (EML) bacterium was recently detected in humans and Ixodes scapularis ticks in Minnesota and Wisconsin. The reservoir for this agent is unknown. To investigate the occurrence of the EML agent, groEL PCR testing and sequencing was performed on blood from small mammals and white-tailed deer that were collected in areas where human and tick infections were previously demonstrated. DNA of the EML agent was detected in two Peromyscus leucopus of 146 small mammals (1.4%); while 181 O. virginianus tested negative. This report provides the first evidence that DNA from the EML agent is found in P. leucopus, the same animal that is a reservoir for Anaplasma phagocytophilum in this region. The role of white-tailed deer remains inconclusive. Further sampling is warranted to understand the spatial and temporal distribution, transmission and maintenance of this pathogen.

KW - Ehrlichia muris

KW - Ehrlichia muris-like (EML) agent

KW - Ehrlichiosis

KW - Peromyscus

UR - https://digitalcommons.georgiasouthern.edu/environ-health-facpubs/33

UR - http://dx.doi.org/10.1016/j.ttbdis.2014.11.006

U2 - 10.1016/j.ttbdis.2014.11.006

DO - 10.1016/j.ttbdis.2014.11.006

M3 - Article

SN - 1877-959X

JO - Ticks and Tick-borne Diseases

JF - Ticks and Tick-borne Diseases

ER -

Detection of Human Pathogenic Ehrlichia muris-like Agent in Peromyscus leucopus

Abstract

Disciplines

Keywords

UN SDGs

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