Determination of genome size and restriction pattern polymorphism of Rickettsia prowazekii and Rickettsia typhi by pulsed field gel electrophoresis

M. E. Eremeeva; V. Roux; D. Raoult

doi:10.1111/j.1574-6968.1993.tb06431.x

Determination of genome size and restriction pattern polymorphism of Rickettsia prowazekii and Rickettsia typhi by pulsed field gel electrophoresis

M. E. Eremeeva, V. Roux, D. Raoult

Biostatistics, Epidemiology, & Environmental Health Sciences

Aix-Marseille Université

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

Pulsed field gel electrophoresis (PFGE) of SmaI, MluI and SalI digested DNA was used to estimate genome size and perform restriction fragment length polymorphism analysis for Rickettsia prowazekii and Rickettsia typhi. We concluded that the genome of R. prowazekii and R. typhi consisted of a single chromosomal DNA. The total length of DNA of R. prowazekii was 1,106±54 kb and of R. typhi was 1,133±44kb. It was possibleto differentiate two strains of R. prowazekii, Breinl and EVir, by PFGE analysis after SalI digestion. Restriction fragment length polymorphism analysis did not reveal intraspecies differences between three human isolates and one Xenopsilla cheopis isolate of R. typhi.

Original language	English
Pages (from-to)	105-112
Number of pages	8
Journal	FEMS Microbiology Letters
Volume	112
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1993.tb06431.x
State	Published - Aug 15 1993

Keywords

Genome size
Pulsed field gel electrophoresis
Rickettsa prowazekii
Rickettsia typhi

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10.1111/j.1574-6968.1993.tb06431.x

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title = "Determination of genome size and restriction pattern polymorphism of Rickettsia prowazekii and Rickettsia typhi by pulsed field gel electrophoresis",

abstract = "Pulsed field gel electrophoresis (PFGE) of SmaI, MluI and SalI digested DNA was used to estimate genome size and perform restriction fragment length polymorphism analysis for Rickettsia prowazekii and Rickettsia typhi. We concluded that the genome of R. prowazekii and R. typhi consisted of a single chromosomal DNA. The total length of DNA of R. prowazekii was 1,106±54 kb and of R. typhi was 1,133±44kb. It was possibleto differentiate two strains of R. prowazekii, Breinl and EVir, by PFGE analysis after SalI digestion. Restriction fragment length polymorphism analysis did not reveal intraspecies differences between three human isolates and one Xenopsilla cheopis isolate of R. typhi.",

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AU - Eremeeva, M. E.

AU - Roux, V.

AU - Raoult, D.

PY - 1993/8/15

Y1 - 1993/8/15

N2 - Pulsed field gel electrophoresis (PFGE) of SmaI, MluI and SalI digested DNA was used to estimate genome size and perform restriction fragment length polymorphism analysis for Rickettsia prowazekii and Rickettsia typhi. We concluded that the genome of R. prowazekii and R. typhi consisted of a single chromosomal DNA. The total length of DNA of R. prowazekii was 1,106±54 kb and of R. typhi was 1,133±44kb. It was possibleto differentiate two strains of R. prowazekii, Breinl and EVir, by PFGE analysis after SalI digestion. Restriction fragment length polymorphism analysis did not reveal intraspecies differences between three human isolates and one Xenopsilla cheopis isolate of R. typhi.

AB - Pulsed field gel electrophoresis (PFGE) of SmaI, MluI and SalI digested DNA was used to estimate genome size and perform restriction fragment length polymorphism analysis for Rickettsia prowazekii and Rickettsia typhi. We concluded that the genome of R. prowazekii and R. typhi consisted of a single chromosomal DNA. The total length of DNA of R. prowazekii was 1,106±54 kb and of R. typhi was 1,133±44kb. It was possibleto differentiate two strains of R. prowazekii, Breinl and EVir, by PFGE analysis after SalI digestion. Restriction fragment length polymorphism analysis did not reveal intraspecies differences between three human isolates and one Xenopsilla cheopis isolate of R. typhi.

KW - Genome size

KW - Pulsed field gel electrophoresis

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KW - Rickettsia typhi

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Determination of genome size and restriction pattern polymorphism of Rickettsia prowazekii and Rickettsia typhi by pulsed field gel electrophoresis

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Keywords

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