Abstract
Fluoromodules are discrete complexes of biomolecules and fluorogenic dyes. Binding of the dyes to their cognate biomolecule partners results in enhanced dye fluorescence. We exploited a previously reported promiscuous binding interaction between a single-chain, variable fragment antibody protein and a family of cyanine dyes to create new protein - dye fluoromodules that exhibit enhanced photostability while retaining high affinity protein - dye binding. Modifications to the dye structure included electronwithdrawing groups that provide resistance to photo-oxidative damage. Low nanomolar equilibrium dissociation constants were found for the new dyes. Fluorescence microscopy illustrates how yeast can be surface-labeled with three different colors based on a single protein and appropriately chosen dyes.
Original language | English |
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Pages (from-to) | 12960-12969 |
Number of pages | 10 |
Journal | Journal of the American Chemical Society |
Volume | 131 |
Issue number | 36 |
DOIs | |
State | Published - Sep 16 2009 |