TY - JOUR
T1 - Imidazolium-dysprosium-based magnetic NanoGUMBOS for isolation of hemoglobin
AU - Cong, Mingyan
AU - Pérez, Rocío L.
AU - Chen, Mi
AU - Chapai, Ramakanta
AU - Jin, Rongying
AU - Vidanapathirana, Punprabhashi
AU - Warner, Isiah M.
N1 - Publisher Copyright:
© 2019
PY - 2019/12/1
Y1 - 2019/12/1
N2 - A novel imidazolium-dysprosium-based magnetic nanomaterial, i.e. [C16mim]5[Dy(SCN)8] nanoGUMBOS (nanomaterials fabricated from a group of uniform material based on organic salts), was prepared using a facile method for selective hemoglobin (Hb) isolation. In this nanomaterial, the imidazolium cation serves as a selective Hb affinity group, while dysprosium contributes paramagnetic properties. Through a combination of the advantages of ionic liquids, magnetic adsorbent, and nanoscale solid phase extraction, [C16mim]5[Dy(SCN)8] nanoGUMBOS exhibit great selectivity toward Hb and a favorable extraction efficiency of 95.4% when 1 mL of 100 μg/mL Hb solution is processed with 0.6 mg of [C16mim]5[Dy(SCN)8] nanoGUMBOS. As the Hb concentration increased to 800 μg/mL, the adsorption capacity approached ∼840 μg/mg. The adsorbed protein is recovered with an elution efficiency of 87% by using 1% SDS solution. This novel nanoGUMBOS solid-phase extraction procedure was successfully applied to selective isolation of Hb from human whole blood and verified using SDS-PAGE. This simple strategy is a novel approach towards fabrication and use of a nanoadsorbent for selective isolation of proteins.
AB - A novel imidazolium-dysprosium-based magnetic nanomaterial, i.e. [C16mim]5[Dy(SCN)8] nanoGUMBOS (nanomaterials fabricated from a group of uniform material based on organic salts), was prepared using a facile method for selective hemoglobin (Hb) isolation. In this nanomaterial, the imidazolium cation serves as a selective Hb affinity group, while dysprosium contributes paramagnetic properties. Through a combination of the advantages of ionic liquids, magnetic adsorbent, and nanoscale solid phase extraction, [C16mim]5[Dy(SCN)8] nanoGUMBOS exhibit great selectivity toward Hb and a favorable extraction efficiency of 95.4% when 1 mL of 100 μg/mL Hb solution is processed with 0.6 mg of [C16mim]5[Dy(SCN)8] nanoGUMBOS. As the Hb concentration increased to 800 μg/mL, the adsorption capacity approached ∼840 μg/mg. The adsorbed protein is recovered with an elution efficiency of 87% by using 1% SDS solution. This novel nanoGUMBOS solid-phase extraction procedure was successfully applied to selective isolation of Hb from human whole blood and verified using SDS-PAGE. This simple strategy is a novel approach towards fabrication and use of a nanoadsorbent for selective isolation of proteins.
KW - Dysprosium
KW - Hemoglobin isolation
KW - Magnetic solid phase extraction
KW - NanoGUMBOS
UR - http://www.scopus.com/inward/record.url?scp=85069588449&partnerID=8YFLogxK
U2 - 10.1016/j.talanta.2019.06.078
DO - 10.1016/j.talanta.2019.06.078
M3 - Article
C2 - 31450390
AN - SCOPUS:85069588449
SN - 0039-9140
VL - 205
JO - Talanta
JF - Talanta
M1 - 120078
ER -