Immunochemical Characterization and Localization of Gastrin/Cholecystokinin-like Immunoreactants in the Dungeness Crab, Cancer Magister

Brett A. Larson; F. W. Scalise; J. R. Reeve; S. R. Vigna

doi:10.1093/icb/22.4.851

Immunochemical Characterization and Localization of Gastrin/Cholecystokinin-like Immunoreactants in the Dungeness Crab, Cancer Magister

Brett A. Larson, F. W. Scalise, J. R. Reeve, S. R. Vigna

Emeriti, Retired & In Memoriam (COSM)

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Abstract

amine in depth the properties of apparent G/CCK-LI in an invertebrate. Preliminary studies revealed the stomach of Canoer to be a rich source of G/CCK-LI. This G/CCKLI cross-reacted with three different antisera specific for the bioactive C-terminus of G/CCK. A relatively large immunoreactive peptide in stomach extracts and a smaller form in the hemolymph were identified by Sephadex G-50 fractionation. Immunocytochemistry revealed specific immunostaining in all gastric epithelial cells and in the gastric cuticle. Also, the exoskeleton contained extractable G/CCK-LI which co-eluted with the hemolymph factor. The major stomach component was purified using gel filtration, ion exchange chromotography, affinity chromatography, and HPLC. Amino acid analysis indicated a peptide of about 20 residues. The C-terminus does not appear to be identical to mammalian G/CCK. If sequence determination confirms these results, this peptide may be a unique member of the G/CCK peptide hormone family. (Supported by grants NSF //PCM 81-04338, USPHS //5 T32 GM0 7413.)

Original language	American English
Title of host publication	American Zoologists
DOIs	https://doi.org/10.1093/icb/22.4.851
State	Published - Nov 1982

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Biology

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title = "Immunochemical Characterization and Localization of Gastrin/Cholecystokinin-like Immunoreactants in the Dungeness Crab, Cancer Magister",

abstract = " amine in depth the properties of apparent G/CCK-LI in an invertebrate. Preliminary studies revealed the stomach of Canoer to be a rich source of G/CCK-LI. This G/CCKLI cross-reacted with three different antisera specific for the bioactive C-terminus of G/CCK. A relatively large immunoreactive peptide in stomach extracts and a smaller form in the hemolymph were identified by Sephadex G-50 fractionation. Immunocytochemistry revealed specific immunostaining in all gastric epithelial cells and in the gastric cuticle. Also, the exoskeleton contained extractable G/CCK-LI which co-eluted with the hemolymph factor. The major stomach component was purified using gel filtration, ion exchange chromotography, affinity chromatography, and HPLC. Amino acid analysis indicated a peptide of about 20 residues. The C-terminus does not appear to be identical to mammalian G/CCK. If sequence determination confirms these results, this peptide may be a unique member of the G/CCK peptide hormone family. (Supported by grants NSF //PCM 81-04338, USPHS //5 T32 GM0 7413.)",

author = "Larson, {Brett A.} and Scalise, {F. W.} and Reeve, {J. R.} and Vigna, {S. R.}",

note = "Abstract, Integrative and Comparative Biology, Volume 22, Issue 4, 1 November 1982, Pages 851-989, https://doi.org/10.1093/icb/22.4.851",

year = "1982",

month = nov,

doi = "10.1093/icb/22.4.851",

language = "American English",

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AU - Larson, Brett A.

AU - Scalise, F. W.

AU - Reeve, J. R.

AU - Vigna, S. R.

N1 - Abstract, Integrative and Comparative Biology, Volume 22, Issue 4, 1 November 1982, Pages 851-989, https://doi.org/10.1093/icb/22.4.851

PY - 1982/11

Y1 - 1982/11

N2 - amine in depth the properties of apparent G/CCK-LI in an invertebrate. Preliminary studies revealed the stomach of Canoer to be a rich source of G/CCK-LI. This G/CCKLI cross-reacted with three different antisera specific for the bioactive C-terminus of G/CCK. A relatively large immunoreactive peptide in stomach extracts and a smaller form in the hemolymph were identified by Sephadex G-50 fractionation. Immunocytochemistry revealed specific immunostaining in all gastric epithelial cells and in the gastric cuticle. Also, the exoskeleton contained extractable G/CCK-LI which co-eluted with the hemolymph factor. The major stomach component was purified using gel filtration, ion exchange chromotography, affinity chromatography, and HPLC. Amino acid analysis indicated a peptide of about 20 residues. The C-terminus does not appear to be identical to mammalian G/CCK. If sequence determination confirms these results, this peptide may be a unique member of the G/CCK peptide hormone family. (Supported by grants NSF //PCM 81-04338, USPHS //5 T32 GM0 7413.)

AB - amine in depth the properties of apparent G/CCK-LI in an invertebrate. Preliminary studies revealed the stomach of Canoer to be a rich source of G/CCK-LI. This G/CCKLI cross-reacted with three different antisera specific for the bioactive C-terminus of G/CCK. A relatively large immunoreactive peptide in stomach extracts and a smaller form in the hemolymph were identified by Sephadex G-50 fractionation. Immunocytochemistry revealed specific immunostaining in all gastric epithelial cells and in the gastric cuticle. Also, the exoskeleton contained extractable G/CCK-LI which co-eluted with the hemolymph factor. The major stomach component was purified using gel filtration, ion exchange chromotography, affinity chromatography, and HPLC. Amino acid analysis indicated a peptide of about 20 residues. The C-terminus does not appear to be identical to mammalian G/CCK. If sequence determination confirms these results, this peptide may be a unique member of the G/CCK peptide hormone family. (Supported by grants NSF //PCM 81-04338, USPHS //5 T32 GM0 7413.)

UR - https://academic.oup.com/icb/article/22/4/851/150258?searchresult=1

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DO - 10.1093/icb/22.4.851

M3 - Chapter

BT - American Zoologists

ER -

Immunochemical Characterization and Localization of Gastrin/Cholecystokinin-like Immunoreactants in the Dungeness Crab, Cancer Magister

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