Immunohistochemical Localization of Multiple Aquaporin Isoforms in Eel Kidney

<p> Presentation given at 11th International Congress on the Biology of Fish, Bi-annual Meeting.</p><p> <a href="https://www2.hw.ac.uk/sls/icbf2014/Abstract%20book%20-%20online%20version.pdf" target="_blank"> Abstracts </a></p><p> Rabbit polyclonal antibodies have been made against eel aquaporin isoforms, AQP1, 1b, 3, 4, 4b, 8aa, 8ab and 10b. These aquaporin isoforms were localized to renal tubules, melano-macrophage-centers, blood vessels or connective tissue using immunohistochemistry and fluorescent and confocal microscopy. Co-localizations of AQP1 and AQP10b (AQPe) were performed with other aquaporin isoforms by labeling these antibodies directly with Dylight 550 and/or Dylight 633 (Pierce/Thermofisher). In marine eel kidney, AQP1 showed co-localization with 79 some AQP1b (AQP1dup), AQP4 and AQP8ab expressing renal tubule segments, although other AQP1b, AQP4 and AQP8ab expressing renal tubule segments showed no AQP1 expression. AQP10b was expressed in connective tissue and basolateral membranes of renal tubule collecting duct cells. Collecting ducts also stained for AQP4 and AQP3, with possible low level expression of AQP1b and AQP8ab. In freshwater eels, apical renal tubule AQP3 expression was found in different tubule segments in comparison to AQP1.</p>