Abstract
<div class="line" id="line-5"> Over the last decade substantial progress has been made in the identification of isoforms of P‐type ATPase subunits. Of the P‐type ATPase characterized, only the four α subunit isoforms of Na,K‐ATPase1 or the two α subunit isoforms of the closely related enzyme H, K‐ATPase2 are known to associate with β subunits. To date, studies investigating the existence of P‐type ATPase β subunit isoforms have resulted in the identification of five β subunit isoforms (designated β1, β2, β3, H,K β, and βb1) in different species.3 However, unlike the Na, K‐ATPase or H, K‐ ATPase α subunit isoforms which share relatively high levels of amino acid homology (>60%), the amino acid sequences of β subunit isoforms share much lower levels of homology, sometimes as low as 30%. The low level of homology of β subunit isoforms makes the task of discovering new isoforms considerably more difficult. Here as part of a wider study investigating the role of ion transporters in various osmoregulatory tissues in euryhaline fish, experiments were designed to determine which P‐type ATPase β subunit isoforms were expressed in the European eel (Anguilla anguilla).</div>
Original language | American English |
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Journal | Annals of the New York Academy of Sciences |
Volume | 834 |
DOIs | |
State | Published - Nov 1997 |
Disciplines
- Biology
Keywords
- ATPase b subunit
- Anguilla anguilla
- Brain
- European Eel
- Isolation
- PCR fragments
- Putative p-type