Microwave Heating of Antibody-functionalized Carbon Nanotubes as a Feasible Cancer Treatment

Brenda Beckler, Ashley Cowan, Nathan Farrar, Amanda Murawski, Alyncia Robinson, Andrew Diamanduros, Karin Scarpinato, Vinoth Sittaramane, Rafael L. Quirino

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Polyclonal antibody/carbon nanotube (CNT) conjugates have been prepared through a di-imide activated amidation process using partially oxidized CNTs. The antibody/nanotube conjugate was used as a microwave absorber with selective affinity for the cell receptor CD44, overexpressed on the surface of PC3 prostate cancer cells. Unlike the most common protocols involving thermic ablation by infrared radiation, microwaves offer a significant higher penetration depth, with instant localized heating when combined with CNTs. The heating rates obtained when multi-walled CNT suspensions in buffer are exposed to microwaves is reported. The antibody/nanotube conjugates were tested in a series of in vitro experiments in which the microwave power and the exposure time were consistently varied. After MTS assays, optimized conditions have been established for the thermal ablation of PC3 cells, while healthy cells remained unharmed. The trends observed in vitro were validated in vivo, using zebrafish embryos as an animal model.

Original languageEnglish
Article number045025
JournalBiomedical Physics and Engineering Express
Volume4
Issue number4
DOIs
StatePublished - Jun 13 2018

Keywords

  • antibodies
  • carbon nanotubes
  • microwaves
  • prostate cancer
  • zebrafish

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