Molecular typing of novel Rickettsia rickettsii isolates from Arizona

Marina E. Eremeeva, Elizabeth Bosserman, Maria Zambrano, Linda Demma, Gregory A. Dasch

Research output: Contribution to book or proceedingConference articlepeer-review

27 Scopus citations

Abstract

Seven isolates of Rickettsia rickettsii were obtained from a skin biopsy, two whole-blood specimens, and from Rhipicephalus sanguineus ticks from eastern Arizona. Molecular typing of seven isolates of R. rickettsii and DNA samples from two other Rh. sanguineus ticks infected with R. rickettsii was conducted by PCR and DNA sequencing of rompA and 12 variable-number tandem repeat regions (VNTRs). All DNA specimens from Arizona were identical to each other and to reference human and Dermacentor andersoni isolates of R. rickettsii from Montana in their rOmpA gene sequences and 10 VNTRs. Two of the twelve VNTRs had differences in the number of repeat sequences in isolates from Arizona compared to those from Montana, thus conferring the novelty of the Rh. sanguineus-associated R. rickettsii.

Original languageEnglish
Title of host publicationCentury of Rickettsiology; Emerging, Reemerging Rickettsioses, Molecular Diagnostics. amd Emerging Veterinary Rickettsioses
PublisherBlackwell Publishing Inc.
Pages573-577
Number of pages5
ISBN (Print)1573316393, 9781573316392
DOIs
StatePublished - Oct 2006

Publication series

NameAnnals of the New York Academy of Sciences
Volume1078
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Keywords

  • Molecular marker
  • Rickettsia rickettsii
  • VNTR

Fingerprint

Dive into the research topics of 'Molecular typing of novel Rickettsia rickettsii isolates from Arizona'. Together they form a unique fingerprint.

Cite this