Muramic Acid in Indoor Air and Dust: Relationship with Home Characteristics

Atin Adhikari; David L. Popham; Christopher Schaffer; Reshmi Indugula; Sergey A. Grinshpun; Tiina Reponen

Muramic Acid in Indoor Air and Dust: Relationship with Home Characteristics

Atin Adhikari, David L. Popham, Christopher Schaffer, Reshmi Indugula, Sergey A. Grinshpun, Tiina Reponen

Biostatistics, Epidemiology, & Environmental Health Sciences

Research output: Contribution to conference › Presentation

Abstract

<div class="line" id="line-136"> <b> Rationale: </b> Muramic acid (bacterial peptidoglycan) is a TLR2 ligand and, similar to other TLR ligands, it could cause either protective or exacerbating effect on allergic asthma depending on the exposure dose. Muramic acid levels were measured in air and dust samples collected from a subgroup of 42 homes from the NIEHS-sponsored Cincinnati Childhood Allergy and Air Pollution Study. The relationships between muramic acid levels and various home characteristics were explored.</div><div class="line" id="line-139"> <br/></div><div class="line" id="line-141"> <b> Methods: </b> Air samples were collected by the Button Inhalable Aerosol Sampler for 24 hours and dust samples were collected by vacuuming of &fllig;ooring. Muramic acid concentrations in the samples were measured by PITC modification, HPLC separation, and detection by absorbance at 254 nm. Temperature and relative humidity (RH) were measured with a portable hygrometer and surface moisture was measured by a protimeter. Environmental relative moldiness index (ERMI) was assessed by qPCR assays of molds in dust samples.</div><div class="line" id="line-144"> <br/></div><div class="line" id="line-146"> <b> Results: </b> The levels of muramic acid in air and dust samples (mean6SD) were 0.962.0 ng/m3 and 21.6631.8 ng/mg, respectively (the corresponding ranges were <LOD–12.3 ng/m3 and 0.1–153.7 ng/mg). Significant correlation (r50.41; p50.006) was observed between air and dust muramic acid levels. In the univariate analysis, number of occupants, temperature, RH, and ERMI were significantly associated with muramic acid levels. However, only ERMI was significantly associated with muramic acid levels in dust and air samples in the multivariate analysis.</div><div class="line" id="line-149"> <br/></div><div class="line" id="line-151"> <b> Conclusions: </b> Muramic acid in indoor air and dust correlate significantly. ERMI values were significantly associated with muramic acid levels in air and dust.</div>

Original language	American English
State	Published - 2013
Event	American Academy of Allergy, Asthma and Immunology Annual Meeting (AAAAI) - San Antonio, TX Duration: Jan 1 2013 → …

Conference

Conference	American Academy of Allergy, Asthma and Immunology Annual Meeting (AAAAI)
Period	01/1/13 → …

DC Disciplines

Environmental Health
Environmental Health and Protection
Environmental Public Health
Public Health

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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@conference{44d482d901cb410ca0cf79b77a49c423,

title = "Muramic Acid in Indoor Air and Dust: Relationship with Home Characteristics",

abstract = " Rationale: Muramic acid (bacterial peptidoglycan) is a TLR2 ligand and, similar to other TLR ligands, it could cause either protective or exacerbating effect on allergic asthma depending on the exposure dose. Muramic acid levels were measured in air and dust samples collected from a subgroup of 42 homes from the NIEHS-sponsored Cincinnati Childhood Allergy and Air Pollution Study. The relationships between muramic acid levels and various home characteristics were explored. Methods: Air samples were collected by the Button Inhalable Aerosol Sampler for 24 hours and dust samples were collected by vacuuming of &fllig;ooring. Muramic acid concentrations in the samples were measured by PITC modification, HPLC separation, and detection by absorbance at 254 nm. Temperature and relative humidity (RH) were measured with a portable hygrometer and surface moisture was measured by a protimeter. Environmental relative moldiness index (ERMI) was assessed by qPCR assays of molds in dust samples. Results: The levels of muramic acid in air and dust samples (mean6SD) were 0.962.0 ng/m3 and 21.6631.8 ng/mg, respectively (the corresponding ranges were <LOD–12.3 ng/m3 and 0.1–153.7 ng/mg). Significant correlation (r50.41; p50.006) was observed between air and dust muramic acid levels. In the univariate analysis, number of occupants, temperature, RH, and ERMI were significantly associated with muramic acid levels. However, only ERMI was significantly associated with muramic acid levels in dust and air samples in the multivariate analysis. Conclusions: Muramic acid in indoor air and dust correlate significantly. ERMI values were significantly associated with muramic acid levels in air and dust.",

author = "Atin Adhikari and Popham, {David L.} and Christopher Schaffer and Reshmi Indugula and Grinshpun, {Sergey A.} and Tiina Reponen",

year = "2013",

language = "American English",

note = "American Academy of Allergy, Asthma and Immunology Annual Meeting (AAAAI) ; Conference date: 01-01-2013",

}

TY - CONF

T1 - Muramic Acid in Indoor Air and Dust: Relationship with Home Characteristics

AU - Adhikari, Atin

AU - Popham, David L.

AU - Schaffer, Christopher

AU - Indugula, Reshmi

AU - Grinshpun, Sergey A.

AU - Reponen, Tiina

PY - 2013

Y1 - 2013

N2 - Rationale: Muramic acid (bacterial peptidoglycan) is a TLR2 ligand and, similar to other TLR ligands, it could cause either protective or exacerbating effect on allergic asthma depending on the exposure dose. Muramic acid levels were measured in air and dust samples collected from a subgroup of 42 homes from the NIEHS-sponsored Cincinnati Childhood Allergy and Air Pollution Study. The relationships between muramic acid levels and various home characteristics were explored. Methods: Air samples were collected by the Button Inhalable Aerosol Sampler for 24 hours and dust samples were collected by vacuuming of &fllig;ooring. Muramic acid concentrations in the samples were measured by PITC modification, HPLC separation, and detection by absorbance at 254 nm. Temperature and relative humidity (RH) were measured with a portable hygrometer and surface moisture was measured by a protimeter. Environmental relative moldiness index (ERMI) was assessed by qPCR assays of molds in dust samples. Results: The levels of muramic acid in air and dust samples (mean6SD) were 0.962.0 ng/m3 and 21.6631.8 ng/mg, respectively (the corresponding ranges were <LOD–12.3 ng/m3 and 0.1–153.7 ng/mg). Significant correlation (r50.41; p50.006) was observed between air and dust muramic acid levels. In the univariate analysis, number of occupants, temperature, RH, and ERMI were significantly associated with muramic acid levels. However, only ERMI was significantly associated with muramic acid levels in dust and air samples in the multivariate analysis. Conclusions: Muramic acid in indoor air and dust correlate significantly. ERMI values were significantly associated with muramic acid levels in air and dust.

AB - Rationale: Muramic acid (bacterial peptidoglycan) is a TLR2 ligand and, similar to other TLR ligands, it could cause either protective or exacerbating effect on allergic asthma depending on the exposure dose. Muramic acid levels were measured in air and dust samples collected from a subgroup of 42 homes from the NIEHS-sponsored Cincinnati Childhood Allergy and Air Pollution Study. The relationships between muramic acid levels and various home characteristics were explored. Methods: Air samples were collected by the Button Inhalable Aerosol Sampler for 24 hours and dust samples were collected by vacuuming of &fllig;ooring. Muramic acid concentrations in the samples were measured by PITC modification, HPLC separation, and detection by absorbance at 254 nm. Temperature and relative humidity (RH) were measured with a portable hygrometer and surface moisture was measured by a protimeter. Environmental relative moldiness index (ERMI) was assessed by qPCR assays of molds in dust samples. Results: The levels of muramic acid in air and dust samples (mean6SD) were 0.962.0 ng/m3 and 21.6631.8 ng/mg, respectively (the corresponding ranges were <LOD–12.3 ng/m3 and 0.1–153.7 ng/mg). Significant correlation (r50.41; p50.006) was observed between air and dust muramic acid levels. In the univariate analysis, number of occupants, temperature, RH, and ERMI were significantly associated with muramic acid levels. However, only ERMI was significantly associated with muramic acid levels in dust and air samples in the multivariate analysis. Conclusions: Muramic acid in indoor air and dust correlate significantly. ERMI values were significantly associated with muramic acid levels in air and dust.

UR - http://www.jacionline.org/article/S0091-6749(12)02723-6/pdf

M3 - Presentation

T2 - American Academy of Allergy, Asthma and Immunology Annual Meeting (AAAAI)

Y2 - 1 January 2013

ER -

Muramic Acid in Indoor Air and Dust: Relationship with Home Characteristics

Abstract

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DC Disciplines

UN SDGs

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