Abstract
We describe the synthesis, characterization, and use of a new photoactive organosilane developed for the photoimmobilization of proteins. Thin films of 2-nitro-5-[11-(trimethoxysilyl)undecyl]oxybenzyl methoxy poly(ethylene glycol) propanoate are characterized using atomic force microscopy, spectroscopic ellipsometry, and attenuated total reflection-Fourier transform infrared spectroscopy. When this organosilane is used to modify silicon substrates, the solvent used during deposition affects the surface concentration and morphology of the resultant film. Although films deposited from either aqueous buffer or anhydrous toluene can be used to attach proteins to surfaces, films deposited from aqueous buffer display 4−6 times less nonspecific protein adsorption that those deposited from toluene. Modification of surfaces with this type of photoactive and protein “rejecting” film should aid in the development of high-resolution protein microarrays.
| Original language | American English |
|---|---|
| Journal | Langmuir |
| Volume | 18 |
| DOIs | |
| State | Published - Jan 1 2001 |
Keywords
- Organosilane films
- Photoactive
- Polyethylene glycol
- Protein immobilization
- Site-specific
DC Disciplines
- Biochemistry, Biophysics, and Structural Biology
- Biology