Protein antigens of genetically related Rickettsia prowazekii strains with different virulence.

N. M. Balayeva; M. E. Eremeeva; V. F. Ignatovich; B. A. Dmitriev; E. B. Lapina; L. S. Belousova

Protein antigens of genetically related Rickettsia prowazekii strains with different virulence.

N. M. Balayeva, M. E. Eremeeva, V. F. Ignatovich, B. A. Dmitriev, E. B. Lapina, L. S. Belousova

Biostatistics, Epidemiology, & Environmental Health Sciences

Russian Academy of Medical Sciences

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The protein antigens of two distinct lines of genetically related strains, namely the nonpathogenic strain E and its virulent revertant EVir and of the standard virulent strain Breinl were compared in SDS-PAGE and immunoblot assay using typhus patient sera and immune rabbit sera. No differences in the polypeptide pattern as detected in SDS-PAGE were found between strain E and EVir; the Breinl strain differed in a 30 kD protein. The high immunogenicity of the protein antigens of E, EVir and Breinl strains was demonstrated by immunoblot assay with human sera, which did not show any differences between the strains studied. Immunoblot analysis with immune rabbit sera to the strain E, EVir, and Breinl showed differences in immunological response to the 70 kD and 60 kD polypeptides of low virulent strain E and those of virulent strains EVir and Breinl.

Original language	English
Pages (from-to)	52-56
Number of pages	5
Journal	Acta Virologica
Volume	36
Issue number	1
State	Published - Jan 1992

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title = "Protein antigens of genetically related Rickettsia prowazekii strains with different virulence.",

abstract = "The protein antigens of two distinct lines of genetically related strains, namely the nonpathogenic strain E and its virulent revertant EVir and of the standard virulent strain Breinl were compared in SDS-PAGE and immunoblot assay using typhus patient sera and immune rabbit sera. No differences in the polypeptide pattern as detected in SDS-PAGE were found between strain E and EVir; the Breinl strain differed in a 30 kD protein. The high immunogenicity of the protein antigens of E, EVir and Breinl strains was demonstrated by immunoblot assay with human sera, which did not show any differences between the strains studied. Immunoblot analysis with immune rabbit sera to the strain E, EVir, and Breinl showed differences in immunological response to the 70 kD and 60 kD polypeptides of low virulent strain E and those of virulent strains EVir and Breinl.",

author = "Balayeva, {N. M.} and Eremeeva, {M. E.} and Ignatovich, {V. F.} and Dmitriev, {B. A.} and Lapina, {E. B.} and Belousova, {L. S.}",

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T1 - Protein antigens of genetically related Rickettsia prowazekii strains with different virulence.

AU - Balayeva, N. M.

AU - Eremeeva, M. E.

AU - Ignatovich, V. F.

AU - Dmitriev, B. A.

AU - Lapina, E. B.

AU - Belousova, L. S.

PY - 1992/1

Y1 - 1992/1

N2 - The protein antigens of two distinct lines of genetically related strains, namely the nonpathogenic strain E and its virulent revertant EVir and of the standard virulent strain Breinl were compared in SDS-PAGE and immunoblot assay using typhus patient sera and immune rabbit sera. No differences in the polypeptide pattern as detected in SDS-PAGE were found between strain E and EVir; the Breinl strain differed in a 30 kD protein. The high immunogenicity of the protein antigens of E, EVir and Breinl strains was demonstrated by immunoblot assay with human sera, which did not show any differences between the strains studied. Immunoblot analysis with immune rabbit sera to the strain E, EVir, and Breinl showed differences in immunological response to the 70 kD and 60 kD polypeptides of low virulent strain E and those of virulent strains EVir and Breinl.

AB - The protein antigens of two distinct lines of genetically related strains, namely the nonpathogenic strain E and its virulent revertant EVir and of the standard virulent strain Breinl were compared in SDS-PAGE and immunoblot assay using typhus patient sera and immune rabbit sera. No differences in the polypeptide pattern as detected in SDS-PAGE were found between strain E and EVir; the Breinl strain differed in a 30 kD protein. The high immunogenicity of the protein antigens of E, EVir and Breinl strains was demonstrated by immunoblot assay with human sera, which did not show any differences between the strains studied. Immunoblot analysis with immune rabbit sera to the strain E, EVir, and Breinl showed differences in immunological response to the 70 kD and 60 kD polypeptides of low virulent strain E and those of virulent strains EVir and Breinl.

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M3 - Article

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Protein antigens of genetically related Rickettsia prowazekii strains with different virulence.

Abstract

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