Regular aerobic exercise-alleviated dysregulation of CAMKIIa carbonylation to mitigate parkinsonism via homeostasis of apoptosis with autophagy

Wenfeng Liu, Rang Fu, Zhiyuan Wang, Shaopeng Liu, Changfa Tang, Li Li

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

This study investigated carbonylation of proteins with oxidative modification profiling in the striatum of aging and Parkinson disease (PD) rats, as well as the long-term effects of regular aerobic exercise on the carbonylation process and the damaging effects of PD vs habitual sedentary behavior. Regular aerobic exercise improved the PD rats' rotational behavior, increased tyrosine hydroxylase expression in both the striatum and substantia nigra pars compacta, and decreased a-synuclein expression significantly. Interestingly, apoptotic nuclei and autophagosomes were increased in the aerobic exercise PD rat striatum. Carbonylated protein Ca2+/calmodulin-dependent protein kinase alpha (CAMKIIa) was present in the middle-aged and aged groups but only in the sedentary, not the exercise, PD rat striatum. Notably, CAMKIIa was characterized by a 4-hydroxynonenal adduct. Regular aerobic exercise upregulated CAMKIIa expression level, activated the CAMK signaling pathway, and promoted the expression of autophagy markers Beclin1 and microtubule-associated proteins 1 A/1B light chain 3II. Aberrant carbonylation of CAMKII initiated age-related changes and might be useful as a potential biomarker of PD. Regular aerobic exercise alleviated protein carbonylation modification of CAMKIIa and regulated the CAMK signaling pathway, thereby affecting and regulating the homeostasis of apoptosis and autophagy in the striatum to alleviate the neurodegenerative process of PD lesions.

Original languageEnglish
Pages (from-to)46-61
Number of pages16
JournalJournal of Neuropathology and Experimental Neurology
Volume79
Issue number1
DOIs
StatePublished - Jan 1 2020

Keywords

  • Aging
  • Apoptosis
  • CAMK signaling pathway
  • Electrospray ionization-quadrupole-time of flight mass spectrometer (ESIQ- TOF-MS)
  • Parkinson disease
  • Posttranslational modification analysis
  • Regular aerobic exercise

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