TY - JOUR
T1 - RNAi screen indicates widespread biological function for human natural antisense transcripts
AU - Faghihi, Mohammad Ali
AU - Kocerha, Jannet
AU - Modarresi, Farzaneh
AU - Engström, Pär G.
AU - Chalk, Alistair M.
AU - Brothers, Shaun P.
AU - Koesema, Eric
AU - St. Laurent, Georges
AU - Wahlestedt, Claes
PY - 2010
Y1 - 2010
N2 - Natural antisense transcripts represent a class of regulatory RNA molecules, which are characterized by their complementary sequence to another RNA transcript. Extensive sequencing efforts suggest that natural antisense transcripts are prevalent throughout the mammalian genome; however, their biological significance has not been well defined. We performed a lossof- function RNA interference (RNAi) screen, which targeted 797 evolutionary conserved antisense transcripts, and found evidence for a regulatory role for a number of natural antisense transcripts. Specifically, we found that natural antisense transcripts for CCPG1 and RAPGEF3 may functionally disrupt signaling pathways and corresponding biological phenotypes, such as cell viability, either independently or in parallel with the corresponding sense transcript. Our results show that the large-scale siRNA screen can be applied to evaluate natural antisense transcript modulation of fundamental cellular events.
AB - Natural antisense transcripts represent a class of regulatory RNA molecules, which are characterized by their complementary sequence to another RNA transcript. Extensive sequencing efforts suggest that natural antisense transcripts are prevalent throughout the mammalian genome; however, their biological significance has not been well defined. We performed a lossof- function RNA interference (RNAi) screen, which targeted 797 evolutionary conserved antisense transcripts, and found evidence for a regulatory role for a number of natural antisense transcripts. Specifically, we found that natural antisense transcripts for CCPG1 and RAPGEF3 may functionally disrupt signaling pathways and corresponding biological phenotypes, such as cell viability, either independently or in parallel with the corresponding sense transcript. Our results show that the large-scale siRNA screen can be applied to evaluate natural antisense transcript modulation of fundamental cellular events.
UR - http://www.scopus.com/inward/record.url?scp=78049308492&partnerID=8YFLogxK
U2 - 10.1371/journal.pone.0013177
DO - 10.1371/journal.pone.0013177
M3 - Article
C2 - 20957177
AN - SCOPUS:78049308492
SN - 1932-6203
VL - 5
JO - PLoS ONE
JF - PLoS ONE
IS - 10
M1 - e13177
ER -