TY - JOUR
T1 - The Balance of Apoptosis and Autophagy via Regulation of the AMPK Signal Pathway in Aging Rat Striatum During Regular Aerobic Exercise
AU - Liu, Wenfeng
AU - Wang, Zhiyuan
AU - Xia, Yan
AU - Kuang, Heyu
AU - Liu, Shaopeng
AU - Li, Li
AU - Tang, Changfa
AU - Yin, Dazhong
PY - 2019/6/27
Y1 - 2019/6/27
N2 - The objective was to analyze the effects of aerobic exercise on aging striatum stress resistance, and the adaptive mechanisms related to neurodegenerative diseases, and the occurrence, and development of neural degeneration. The 10-weeks of regular moderate-intensity aerobic exercise intervention were carried out in the aerobic exercise runner Sprague-Dawley rats. Apoptotic nuclei appeared in the striatum of aged rats, showing a tendency to relate to aging. The apoptotic index of the striatum in young, middle-aged, and old-aged rats of the aerobic exercise groups increased by 205.56%, 57%, and 68.24%. Autophagy markers Beclin l and LC 3-II expression, AMPKα1 and pAMPKα1 expression increased significantly in all age-exercise groups. The ratio of AMPKα1/pAMPKα1 increased after exercise, and the tendency of exercise to alter autophagy and cell apoptosis increased with aging. Then SirT2 mRNA was significantly upregulated in the aerobic exercise runner groups. In conclusion, we showed that the balance of autophagy and apoptosis were closely regulated by regular aerobic exercise, which affected the development of aging, and via regulation of the AMPK/SirT2 signaling pathway.
AB - The objective was to analyze the effects of aerobic exercise on aging striatum stress resistance, and the adaptive mechanisms related to neurodegenerative diseases, and the occurrence, and development of neural degeneration. The 10-weeks of regular moderate-intensity aerobic exercise intervention were carried out in the aerobic exercise runner Sprague-Dawley rats. Apoptotic nuclei appeared in the striatum of aged rats, showing a tendency to relate to aging. The apoptotic index of the striatum in young, middle-aged, and old-aged rats of the aerobic exercise groups increased by 205.56%, 57%, and 68.24%. Autophagy markers Beclin l and LC 3-II expression, AMPKα1 and pAMPKα1 expression increased significantly in all age-exercise groups. The ratio of AMPKα1/pAMPKα1 increased after exercise, and the tendency of exercise to alter autophagy and cell apoptosis increased with aging. Then SirT2 mRNA was significantly upregulated in the aerobic exercise runner groups. In conclusion, we showed that the balance of autophagy and apoptosis were closely regulated by regular aerobic exercise, which affected the development of aging, and via regulation of the AMPK/SirT2 signaling pathway.
KW - AMPK signaling pathway
KW - Aging
KW - Apoptosis
KW - Autophagy
KW - Regular aerobic exercise
KW - Striatum
UR - https://digitalcommons.georgiasouthern.edu/health-kinesiology-facpubs/174
UR - https://doi.org/10.1016/j.exger.2019.110647
U2 - 10.1016/j.exger.2019.110647
DO - 10.1016/j.exger.2019.110647
M3 - Article
SN - 0531-5565
VL - 124
JO - Experimental Gerontology
JF - Experimental Gerontology
ER -