Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea: A case report

Emine E. Alieva; Muniver T. Gafarova; Eugene I. Bondarenko; Igor A. Dovgan; Irina I. Osiptchuk; Marina E. Eremeeva

doi:10.1016/j.imj.2023.09.004

Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea: A case report

Emine E. Alieva
, Muniver T. Gafarova
, Eugene I. Bondarenko
, Igor A. Dovgan
, Irina I. Osiptchuk
, Marina E. Eremeeva

Biostatistics, Epidemiology & Environmental Health Sciences

Ministry of Defence of the Russian Federation
V.I. Vernadsky Crimean Federal University
Vector-Best
Saki Regional Hospital
Georgia Southern University

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

Mediterranean spotted fever (MSF) has been diagnosed clinically in the Crimean Peninsula since the 1930s. We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever, maculopapular rash, and eschar. Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes. The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7. This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.

Original language	English
Pages (from-to)	338-342
Number of pages	5
Journal	Infectious Medicine
Volume	2
Issue number	4
DOIs	https://doi.org/10.1016/j.imj.2023.09.004
State	Published - Oct 27 2023

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Scopus Subject Areas

Immunology and Allergy
General Immunology and Microbiology
Microbiology (medical)
Infectious Diseases

Keywords

Eschar
Mediterranean spotted fever
PCR
Rickettsia conorii
Swab

Access to Document

10.1016/j.imj.2023.09.004

Cite this

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title = "Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea: A case report",

abstract = "Mediterranean spotted fever (MSF) has been diagnosed clinically in the Crimean Peninsula since the 1930s. We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever, maculopapular rash, and eschar. Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes. The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7. This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.",

keywords = "Eschar, Mediterranean spotted fever, PCR, Rickettsia conorii, Swab",

author = "Alieva, \{Emine E.\} and Gafarova, \{Muniver T.\} and Bondarenko, \{Eugene I.\} and Dovgan, \{Igor A.\} and Osiptchuk, \{Irina I.\} and Eremeeva, \{Marina E.\}",

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day = "27",

doi = "10.1016/j.imj.2023.09.004",

language = "English",

volume = "2",

pages = "338--342",

journal = "Infectious Medicine",

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TY - JOUR

T1 - Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea

T2 - A case report

AU - Alieva, Emine E.

AU - Gafarova, Muniver T.

AU - Bondarenko, Eugene I.

AU - Dovgan, Igor A.

AU - Osiptchuk, Irina I.

AU - Eremeeva, Marina E.

PY - 2023/10/27

Y1 - 2023/10/27

N2 - Mediterranean spotted fever (MSF) has been diagnosed clinically in the Crimean Peninsula since the 1930s. We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever, maculopapular rash, and eschar. Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes. The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7. This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.

AB - Mediterranean spotted fever (MSF) has been diagnosed clinically in the Crimean Peninsula since the 1930s. We describe the recent illness of an elderly patient from Crimea who had developed a classic triad of MSF symptoms consisting of fever, maculopapular rash, and eschar. Clinical diagnosis of rickettsiosis was confirmed using real-time PCR and sequencing of 4 Rickettsia protein genes. The strain causing clinical illness was characterized as Rickettsia conorii subspecies conorii Malish 7. This report corroborates the utility of eschar swab material as a source of DNA for PCR-based diagnostics that enables timely patient treatment and management.

KW - Eschar

KW - Mediterranean spotted fever

KW - PCR

KW - Rickettsia conorii

KW - Swab

UR - https://www.scopus.com/pages/publications/85178182503

U2 - 10.1016/j.imj.2023.09.004

DO - 10.1016/j.imj.2023.09.004

M3 - Article

C2 - 38205177

AN - SCOPUS:85178182503

SN - 2097-0684

VL - 2

SP - 338

EP - 342

JO - Infectious Medicine

JF - Infectious Medicine

IS - 4

ER -

Use of eschar swab DNA to diagnose Rickettsia conorii subspecies conorii infection in Crimea: A case report

Abstract

UN SDGs

Scopus Subject Areas

Keywords

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